Delivery of dna using this technique is simple ...

11-G00255_ID2139_rev. PDF

genetic engineering invented at ORNL facilitates the delivery of DNA buy strattera online into cells using ultrasound to penetrate the plasma membrane of cells. Delivery of DNA using this technique is simple, fast, inexpensive and provides a versatile method of gene transfer. Existing methods for delivering DNA all have significant drawbacks, including substantial damage to the cell membrane. These traditional methods require repeated rounds of washing and other treatments, to make DNA, causing the protocol complex and difficult. The invention provides ORNL sonoporation based method using ultrasonic frequencies effectively alter the permeability of the plasma membrane of cells to insert the selected connection. The method can be universally used for delivery of nucleic acids, proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules and nanoparticles gram-positive bacteria, including Bacillus, Streptococcus, Acetobacterium and Clostridium. Simple, fast, easy and economical >> << process because mechanical method, the invention may be well suited for different types of cells and tissues

health, plant biology, and biopharmaceutical research

Gene therapy holds great promise for treating cancer, HIV and other hereditary and infectious diseases

Yunfeng Yang and Lee Youngchao,

Transformation of Gram-positive bacteria on Sonoporation, U.S. Patent 20100196983, filed Jan. 29, 2010. .